FIGURE

Fig. 11

ID

ZDB-FIG-160217-15

Publication

Yokota et al., 2015 - Endothelial Ca(2+) oscillations reflect VEGFR signaling-regulated angiogenic capacity in vivo

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Fig. 11

Dll4 does not regulate Ca²⁺ oscillations in stalk cells after they completely migrate out of the DA.

(A) 3D-rendered time-sequential images of Tg(fli1:Gal4FF);(UAS:GCaMP7a);(fli1:H2B-mC) embryos after stalk cell budding from the DA injected with dll4 MO (27 ss). Green and orange arrowheads indicate tip and stalk cells, respectively. (B) The fluorescence changes in GCaMP7a (ΔF/F₀) of individual ECs from A indicated by arrowheads at the left panel are shown as a graph. (C) Ca²⁺ oscillation frequency (left) and mean ΔF/F₀ (right) in tip cells, stalk cells, and other ECs within the DA in control MO- or dll4 MO-injected embryos after stalk cell budding from the DA as illustrated at the left panel. (n ≥ 9). Scale bar, 10 mm in A.

Expression Data

Genes:	GCaMP mCherry
Fish:	ncv31Tg; ubs4Tg; zf415Tg + MO3-dll4
Knockdown Reagent:	MO3-dll4
Anatomical Terms:	blood vessel endothelial cell cytoplasm endothelial cell nucleus endothelial tip cell cytoplasm intersegmental vessel cytoplasm
Stage Range:	26+ somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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