FIGURE

Fig. 9

ID

ZDB-FIG-160215-26

Publication

Yokota et al., 2015 - Endothelial Ca(2+) oscillations reflect VEGFR signaling-regulated angiogenic capacity in vivo

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Fig. 9

Dll4 is involved in suppressing Ca²⁺ responses in ECs adjacent to tip cells.

(A) The number of Ca²⁺-oscillating ECs at each somite boundary of the embryo injected with control MO or dll4 MO was quantified as in Figure 4A. (B) 3D-rendered time-sequential images of Tg(fli1:Gal4FF);(UAS:GCaMP7a);(fli1:H2B-mC) embryos during tip cell budding injected with dll4 MO (24 ss). Green and blue arrowheads indicate two neighboring Ca²⁺-oscillating ECs, both of which are budding from the DA. (C) The fluorescence changes in GCaMP7a (ΔF/F₀) of individual ECs from B indicated by arrowheads at the left panel are shown as a graph. (D) Ca²⁺ oscillation frequency (left) and mean ΔF/F₀ (right) in budding ECs and other ECs within the DA in dll4 morphants during tip cell budding at 24–27 ss as illustrated at the left panel (n e 13). Scale bar, 10 mm in B. ***p < 0.001. DA, dorsal aorta.

Expression Data

Genes:	GCaMP mCherry
Fish:	ncv31Tg; ubs4Tg; zf415Tg + MO3-dll4
Knockdown Reagent:	MO3-dll4
Anatomical Terms:	blood vessel endothelial cell cytoplasm endothelial cell nucleus endothelial tip cell
Stage Range:	20-25 somites to 26+ somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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