FIGURE

Fig. 5

ID

ZDB-FIG-160208-35

Publication

Shen et al., 2015 - Down-regulation of msrb3 and destruction of normal auditory system development through hair cell apoptosis in zebrafish

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Fig. 5

Msrb3 morphant apoptotic hair cells. (A,B) TUNEL assay data. In 3 dpf larvae, msrb3 morphants had more hair cell apoptosis than mismatch controls. Scale bars, 5 µm. (C) Statistical analysis of apoptotic hair cells per neuromast in controls and msrb3 morphants, “n” represents neuromasts number. Error bars are s.d. ***P<0.0001. (D,E) Zebrafish embryos injected with msrb3 morpholinos (In2MO, Ex2MO) or co-injected with msrb3 morpholinos and p53MO-AO staining to detect whole larval apoptosis signal at 24 hpf. There was no difference between msrb3 morphants and p53MO co-injected larvae. The p53 off-target effect was ruled out. Scale bars, 200 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-msrb3 AB + MO1-msrb3 + MO4-tp53 AB + MO2-msrb3 AB + MO2-msrb3 + MO4-tp53
Knockdown Reagents:	MO1-msrb3 MO2-msrb3 MO4-tp53
Observed In:	apoptotic process neuromast hair cell apoptotic process
Stage Range:	Prim-5 to Protruding-mouth

Phenotype Detail

Acknowledgments

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