FIGURE

Fig. S2

ID

ZDB-FIG-151214-50

Publication

Roxo-Rosa et al., 2015 - The zebrafish Kupffer's vesicle as a model system for the molecular mechanisms by which the lack of Polycystin-2 leads to stimulation of CFTR

Other Figures

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Fig. S2

Ouabain effect on KV volume. Live-scans of the whole KV of 10–11 s.s. ras:GFP transgenic embryos. The middle focal plan along the XY axis and the respective orthogonal views (along XZ and YZ axes) are shown for the most representative 5 µM ouabain treated WT (A) and pkd2-morphant (B) embryos. KV^volume is indicated in µm³ and in picoL. Scale bars: 10 µm. (C) Estimated KV volume (µm³) for WT (n = 16), pkd2-mismatch MO (n = 12), 5 µM ouabain treated WT (n = 13) and pkd2-morphant (n = 15) embryos. Average values and the respective s.d. are indicated. * and **, significantly different from pkd2-morphants (p < 0.05 and p < 0.0001, respectively).

Expression Data

Gene:	EGFP
Fish:	vu119Tg vu119Tg + MO3-pkd2 + MO9-pkd2
Condition:	chemical treatment: ouabain
Knockdown Reagents:	MO3-pkd2 MO9-pkd2
Anatomical Term:	Kupffer's vesicle epithelial cell
Stage:	10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	vu119Tg vu119Tg + MO3-pkd2 + MO9-pkd2
Condition:	chemical treatment: ouabain
Knockdown Reagents:	MO3-pkd2 MO9-pkd2
Observed In:	Kupffer's vesicle
Stage:	10-13 somites

Phenotype Detail

Acknowledgments

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