FIGURE

Fig. 2

ID

ZDB-FIG-151214-27

Publication

Dutta et al., 2015 - nlz1 Is required for cilia formation in zebrafish embryogenesis

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Fig. 2

Nlz1 is important for left–right patterning in zebrafish. Zebrafish embryos were injected with control MO (A), (D), and (G) or with 2 ng of Nlz1 MO (B), (B2), (E), (E′), (H), and (H′). Embryos were hybridized in situ with laterality marker genes spw (A), (B), (B′), lft2 (D), (E), and (E2), and cmlc2 (G), (H), (H′) RNA probes. Quantification of expression patterns of spw (C), lft2 (F), and cmlc2 (I) in MO (spw, n=85; lft2, n=110; cmlc2, n=75) and MO+mRNA injected embryos (spw, n=75; lft2,n=100; cmlc2, n=55). (A), (B), (B′), (D), (E), (E′) dorsal view at 18–20S stage, (G), (H), (H′) frontal view at 28 h. Scale bar 100 µm. Ab: absent; Bi: bilateral, M: midline; R: right; L: left.

Expression Data

Genes:	lft2 myl7 spaw
Fish:	WT WT + MO1-znf703
Knockdown Reagent:	MO1-znf703
Anatomical Terms:	heart tube lateral plate mesoderm left side lateral plate mesoderm right side
Stage Range:	14-19 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-znf703
Knockdown Reagent:	MO1-znf703
Observed In:	determination of heart left/right asymmetry determination of left/right symmetry heart tube lateral plate mesoderm right side
Stage Range:	14-19 somites to Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 406(2), Dutta, S., Sriskanda, S., Boobalan, E., Alur, R.P., Elkahloun, A., Brooks, B.P., nlz1 Is required for cilia formation in zebrafish embryogenesis, 203-11, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.