Fig. S1
Morpholino designs and inhibitory efficacies. (A) Schematic representation of the coding sequence of the zebrafish Vegfba and Vegfbb genes. Morpholinos designed in this study were indicated. (B) Whole-mount immunohistochemical staining with an anti-Myc antibody of 24-hpf zebrafish embryos injected with 100 ng Vegfba:myc or Vegfbb:myc mRNAs with or without the corresponding ATG-blocking morpholinos. (Scale bar, 100 µm.) (C) Nucleotide sequences of Vegfb morpholinos and their targets. Lowercase letters in the targets indicate noncoding bases. (D) Quantification of anti-myc reactivity signals in 24-hpf zebrafish embryos injected with 100 ng Vegfba:myc or Vegfbb:myc mRNA with or without the corresponding ATG-blocking morpholinos (average: n = 18). **P < 0.01; ***P < 0.001. |