FIGURE

Fig. 3

ID

ZDB-FIG-151117-3

Publication

Wang et al., 2015 - Irf4 Regulates the Choice between T Lymphoid-Primed Progenitor and Myeloid Lineage Fates during Embryogenesis

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Fig. 3

Homing Process Is Disturbed due to the Reduced Expression of ccr9a

(A) Confocal imaging of coro1a:EGFP/lyz:DsRed embryos showing no coro1a:EGFP cells in the thymus (white circle) in irf4a morphants. Scale bar, 40 µm.

(B) Number of coro1a:EGFP cells entering the thymus every 30 min in the control and irf4a morphants (n = 6) during the homing process. p < 0.05.

(C) Quantification of embryos with coro1a:EGFP cells (>5) in the thymus of the control (n = 88) and irf4a morphants (n = 92) at 3.5 dpf.

(D) Expression of foxn1 in the control and irf4a morphants at 3 dpf.

(E) Expression of ccl25a and ccl25b in the control and irf4a morphants at 3 dpf.

(F) Expression of ccr9a in the control and irf4a morphants at 3 dpf. Red arrows indicate the thymus or perithymic region.

(G) Expression of cmyb, ikaros, and ccr9a in the CHT of the control and irf4a morphants at 2 dpf. Red arrows indicate hematopoietic progenitors expressing cmyb, ikaros, or ccr9a in the CHT region.

(H) qPCR analysis of cmyb, ikaros, and ccr9a in the control and irf4a morphants at 2 dpf. ns, not significant. p < 0.01. All data are mean ± SD.

See also Figure S3 and Movie S1.

Expression Data

Genes:	ccl25a ccl25b ccr9a DsRed2 EGFP foxn1 ikzf1 myb
Fish:	WT WT + MO1-irf4a hkz04tTg; nz50Tg hkz04tTg; nz50Tg + MO1-irf4a
Knockdown Reagent:	MO1-irf4a
Anatomical Terms:	intermediate cell mass of mesoderm intermediate cell mass of mesoderm hematopoietic multipotent progenitor cell neutrophil thymus thymus primordium thymus primordium neutrophil
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-irf4a hkz04tTg; nz50Tg + MO1-irf4a
Knockdown Reagent:	MO1-irf4a
Observed In:	intermediate cell mass of mesoderm lymphoid lineage cell migration into thymus thymus thymus hematopoietic stem cell migration thymus primordium
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 34(6), Wang, S., He, Q., Ma, D., Xue, Y., Liu, F., Irf4 Regulates the Choice between T Lymphoid-Primed Progenitor and Myeloid Lineage Fates during Embryogenesis, 621-31, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell