Fig. 6
- ID
- ZDB-FIG-151106-6
- Publication
- Sun et al., 2015 - BNIP-H Recruits the Cholinergic Machinery to Neurite Terminals to Promote Acetylcholine Signaling and Neuritogenesis
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Knockdown of Bnip-h Causes Abnormal Axon Growth of Motor Neurons in Zebrafish through Cholinergic Signaling (A–D) The axon growth of motor neurons is regulated by Bnip-h, Acl and Chat. The axons of motor neurons were stained with anti-synaptotagmin-2 antibody (Znp-1) and imaged with confocal microscope. Images are maximum intensity projections of z stacks. Total neurite length per myotome (excluding myoseptum) was calculated. Five fishes for each condition were quantified. Error bars represent SD. p < 0.05; p < 0.001. Scale bar represents 100 µm. (E and F) Bnip-h regulates axon growth of primary motor neurons through muscarinic receptors and MAPK pathway. Primary motor neurons were isolated from 18 somite larvae, and treated with indicated chemicals for 48 hr. Quantification was done as in Figure 1B. n = 30–50 cells/category from three experiments. Error bars represent SD. p < 0.01; p < 0.001; N. S., not significant. Scale bar represents 50 µm. See also Figure S6. |
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| Stage: | Protruding-mouth |
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| Stage: | Protruding-mouth |
Reprinted from Developmental Cell, 34(5), Sun, J., Pan, C.Q., Chew, T.W., Liang, F., Burmeister, M., Low, B.C., BNIP-H Recruits the Cholinergic Machinery to Neurite Terminals to Promote Acetylcholine Signaling and Neuritogenesis, 555-68, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell