FIGURE

Fig. 5

ID

ZDB-FIG-151102-32

Publication

Ghaye et al., 2015 - Progenitor potential of nkx6.1-expressing cells throughout zebrafish life and during beta cell regeneration

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Fig. 5

ascl1b and nkx6.1 are regulated in an opposite way by the Notch signaling pathway. a–f WISH on wild-type (WT) embryos (a, c, e) or mind bomb (mib^-/-) mutants (b, d, f) with ascl1b (a, b) or nkx6.1 (c–f) probes. Brackets delimit the pancreatic domain. g, h Immunodetection of GFP and endogenous Nkx6.1 in 5-dpf Tg(ascl1b:eGFP-creER ^T2 ) embryos treated from 3 to 5 dpf with DMSO (g) or with the Notch signaling inhibitor, LY411575 (h). i–n WISH with ascl1b (i, j) or nkx6.1 (k, l) probes or immunodetection of endogenous Nkx6.1 (m, n) of double-transgenic Tg(hsp70:Gal4) x Tg(UAS:NICD) embryos (j, l, n) or of control simple-transgenic embryos (i, k, m), both heat-shocked at 11 hpf for 20 min. Lateral views (a–f, k, l) or ventral views (g–j, m, n) of embryos of the indicated stages with the anterior to the left. Scale bars = 40 µm

Expression Data

Genes:	ascl1b EGFP nkx6.1
Antibody:	Ab2-nkx6
Fish:	AB mib1^ta52b/ta52b ulg006Tg (AB) kca3Tg; kca4Tg
Conditions:	chemical treatment: LY-411575 heat shock
Anatomical Terms:	endocrine pancreas nervous system pancreas primordium pancreatic bud pancreatic duct
Stage Range:	10-13 somites to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mib1^ta52b/ta52b ulg006Tg (AB) kca3Tg; kca4Tg
Conditions:	chemical treatment: LY-411575 heat shock
Observed In:	endocrine pancreas nervous system pancreas primordium pancreatic bud pancreatic duct
Stage Range:	10-13 somites to Day 5

Phenotype Detail

Acknowledgments

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