FIGURE

Fig. S2

ID

ZDB-FIG-151102-38

Publication

Ghaye et al., 2015 - Progenitor potential of nkx6.1-expressing cells throughout zebrafish life and during beta cell regeneration

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Fig. S2

The bacterial artificial chromosome (BAC) reporter line Tg (ascl1b:eGFP-creER T2 ) mirrors the expression of the endogenous ascl1b gene. A Schematic representation of the 61 to +89 kb ascl1b:eGFP- 2A-creER T2 BAC transgene. By BAC recombineering using galK selection, the eGFP-2A-creER T2 cassette is introduced into exon 1, replacing the beginning of the ascl1b open reading frame (aa 1 to 163). B Epifluorescence microscopy images of the immunodetection of GFP in 17-hpf Tg(ascl1b:eGFP-creER T2 ) embryos. C, D Visible WISH showing expression of endogenous ascl1b in a wild-type (WT) embryo (C) and of GFP in Tg(ascl1b:eGFP-creER T2 ) embryos (D) at 15 hpf. Lateral views with the anterior part to the left. P pancreas.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Biol.