Fig. 8

Knockdown of klf8 results in decreased met expression in the rhombomere 1 (R1) and tectum, and co-injection of met mRNA rescues development of Purkinje cells in klf8 morphants. (A–D) Reduced met expression in the tectum (arrowhead) and R1 (asterisk) is observed at 24 hpf in embryos injected with klf8-MO1^atg (C) or co-injected with klf8-MO^AC and klf8-MO^DO (D), as compared to WT embryos (A) or embryos injected with klf8-4mmMO1 (B). (E) Percentage of embryos of the indicated backgrounds with decreased met expression relative to WT embryos. (F–I) Decreased met expression in the tectum (arrowhead) and R1 (asterisk) of klf8 morphants (G) relative to WT (F) is rescued by co-injection with klf8 mRNA (I), but not LacZ mRNA (H). (J) Percentage of embryos of the indicated backgrounds with decreased met expression relative to WT embryos. (K–N) Perturbed Purkinje cell development in klf8 morphants (L) as compared to WT embryos (K) can be rescued by co-injection of met mRNA (N), but not LacZ mRNA (M). (O) Percentage of embryos of the indicated backgrounds with aberrant Purkinje cell development as compared to WT embryos. (A–D) lateral views; (F–I, K–N) dorsal views. Significance was determined using Student′s t-test. **p < 0.01, ***p < 0.001. Error bars indicate standard errors. R1, rhombomere 1; te, tectum.