FIGURE

Fig. 8

ID

ZDB-FIG-150916-37

Publication

Patil et al., 2015 - scaRNAs Regulate Splicing and Vertebrate Heart Development

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Fig. 8

Splice isoform changes after targeting scarna1. Treating zebrafish embryos with anti-scarna1 morpholino causes changes in exon retention of cardiac regulatory genes. 13 of 39 members of the Wnt family have significant changes in exon retention after treatment with antisense morpholino (assessed by RNA-Seq and qRT-PCR, values shown are from qRT-PCR data quantifying the variable exon). The misMO oligo is a negative control that differs from the primary morpholino by 5 nucleotides in the critical binding site. The gene name is shown on the horizontal axis and the numbers represent the start position of the exon. *Significantly different from the WT, p < 0.05.

Expression Data

Genes:	dot1l fzd3b kctd15a mllt10 sfrp1a vangl2 wnt4 wnt4b wnt8a wnt8b wnt10a wnt10b
Fish:	AB AB + MO1-scarna1
Knockdown Reagent:	MO1-scarna1
Anatomical Term:	whole organism
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-scarna1 AB + MO1-snord94
Knockdown Reagents:	MO1-scarna1 MO1-snord94
Observed In:	regulation of alternative mRNA splicing, via spliceosome
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Biochimica et biophysica acta. Molecular basis of disease, 1852(8), Patil, P., Kibiryeva, N., Uechi, T., Marshall, J., O'Brien, J.E., Artman, M., Kenmochi, N., Bittel, D.C., scaRNAs Regulate Splicing and Vertebrate Heart Development, 1619-29, Copyright (2015) with permission from Elsevier. Full text @ BBA Molecular Basis of Disease