FIGURE

Fig. 2

ID

ZDB-FIG-150526-2

Publication

Ye et al., 2015 - glucagon is essential for alpha cell transdifferentiation and beta cell neogenesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

δ cells, but not regenerating β cells, are lineage marked by sst2:Cre. (A-A′′′) Merged and single-channel images of 5-dpf Tg(sst2:Cre); Tg(hs:CSH); Tg(ins:Flag-NTR) islets that were heat shocked at 3dpf and stained for GFP (green), insulin (red) and somatostatin (white). Somatostatin⁺ δ cells are labeled by H2B-GFP. (B-C′′′) Merged and single-channel images of 5-dpf non-ablated (B) or 1dpa (C) Tg(sst2:Cre); Tg(hs:CSH); Tg(ins:Flag-NTR) islets that were heat shocked at 3dpf and stained for GFP (green), insulin (red) and DNA (blue). (D) Quantification of total somatostatin⁺, somatostatin⁺/H2B-GFP⁺, insulin⁺, and insulin⁺/H2B-GFP⁺ cells in non-ablated 5-dpf Tg(sst2:Cre); Tg(hs:CSH); Tg(ins:Flag-NTR) islets. 45% of somatostatin⁺ cells and 1.5% of insulin⁺ cells are marked by sst2:Cre (n=15). (E) Quantification of insulin⁺ and insulin⁺/H2B-GFP⁺ cells in 5-dpf regenerating islets. 1% of insulin⁺ cells are marked by sst2:Cre (n=9).

Expression Data

Genes:	GFP ins
Antibody:	ab2-sst
Fish:	s923Tg; s950Tg; s963Tg
Conditions:	heat shock chemical treatment: pharmaceutical, heat shock
Anatomical Terms:	pancreatic B cell pancreatic D cell
Stage Range:	Day 5 to Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development