ZFIN Image: Ye et al., 2015, Fig. 2

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Figure Caption

Fig. 2

δ cells, but not regenerating β cells, are lineage marked by sst2:Cre. (A-A′′′) Merged and single-channel images of 5-dpf Tg(sst2:Cre); Tg(hs:CSH); Tg(ins:Flag-NTR) islets that were heat shocked at 3dpf and stained for GFP (green), insulin (red) and somatostatin (white). Somatostatin⁺ δ cells are labeled by H2B-GFP. (B-C′′′) Merged and single-channel images of 5-dpf non-ablated (B) or 1dpa (C) Tg(sst2:Cre); Tg(hs:CSH); Tg(ins:Flag-NTR) islets that were heat shocked at 3dpf and stained for GFP (green), insulin (red) and DNA (blue). (D) Quantification of total somatostatin⁺, somatostatin⁺/H2B-GFP⁺, insulin⁺, and insulin⁺/H2B-GFP⁺ cells in non-ablated 5-dpf Tg(sst2:Cre); Tg(hs:CSH); Tg(ins:Flag-NTR) islets. 45% of somatostatin⁺ cells and 1.5% of insulin⁺ cells are marked by sst2:Cre (n=15). (E) Quantification of insulin⁺ and insulin⁺/H2B-GFP⁺ cells in 5-dpf regenerating islets. 1% of insulin⁺ cells are marked by sst2:Cre (n=9).

Figure Data

Expression / Labeling details

Acknowledgments

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