Fig. 4

Transcription Is Not Largely Affected in log (med14) Mutants

(A–D) Expression of opn1sw1, initiated by 2.5 dpf in WT embryos, reaches WT levels in log mutant embryos by 4.0 dpf.

(E) Tg(hsp70:EGFP) log mutant embryos heat shocked at 3.5 dpf show robust GFP signal.

(F–H′) log mutant cells transplanted into WT hosts (traced using a β-actin:EGFP transgene) are evident in 15 dpf hosts (including in the semicircular canals, yellow arrowhead in F) and survive until 2 ypf (years post-fertilization, yellow arrowheads).

(I) Summary of results of microarray analysis on cDNA from 2.25 dpf WT versus log mutant embryos.

(J) Quantification of mRNA per 0.1mg total RNA at 3.25 dpf from WT and log mutant embryos.

(K) Normalized qPCR values for med14 expression in mutant relative to WT control embryos (p < 0.05 using the one-tailed unpaired Student’s t test).

(L) Mean normalized expression (MNE) of β-actin in WT and MUT embryos at 2.25 dpf calculated using a universal reference approach. No significant difference (p = 0.63) was observed between WT (0.107 ± 0.00380) and log MUT (0.123 ± 0.0297) samples (n = 3) by one-tailed unpaired Student’s t test. For (J)–(L), three biological replicates of 450 (J) or 10 (K and L) embryos were used.