FIGURE

Fig. 3

ID

ZDB-FIG-150526-16

Publication

Burrows et al., 2015 - An In Vivo Requirement for the Mediator Subunit Med14 in the Maintenance of Stem Cell Populations

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Fig. 3

Expression and Timing Requirement of med14 for Development

(A) Worsening of the log mutant phenotype by injection of morpholino (MO) targeting med14 (red arrowhead denotes somite defect).

(A′ and A′′) Somite structure of 3.5 dpf rhodamine phalloidin (RhPh) stained WT and MO-injected mutant embryos. Defects in muscle fiber patterns of mutant embryos injected with MO are shown (yellow arrowheads).

(B–D) RNA ISH analysis shows that med14 is broadly expressed at the one- and two-cell stage and at 12 hpf (“WT/MUT” denotes unknown genotype). At 24 hpf, broad med14 expression is undetectable in zygotic med14 mutants.

(E–K) Temporal rescue of the log mutant phenotype using Tg(hsp70:med14, α-crystallin:EGFP), with initial heat shock performed at the specified time, and then every 12 hr following until 5 dpf. Scale bars, 0.5 mm.

Expression Data

Gene:	med14
Fish:	WT med14^m628/m628
Anatomical Terms:	head trunk anterior region whole organism
Stage Range:	2-cell to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	med14^m628/m628 + MO1-med14 hsc10Tg med14^m628/m628; hsc10Tg
Condition:	heat shock
Knockdown Reagent:	MO1-med14
Observed In:	embryonic organ development myotome skeletal muscle cell whole organism
Stage Range:	Protruding-mouth to Day 5

Phenotype Detail

Acknowledgments

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