FIGURE

Fig. 2

ID

ZDB-FIG-150526-15

Publication

Burrows et al., 2015 - An In Vivo Requirement for the Mediator Subunit Med14 in the Maintenance of Stem Cell Populations

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Fig. 2

Logelei Results from a Mutation in med14

(A) Recombination frequency mapping results. Screening of 1,800 map-cross mutant embryos with markers Z45039 and Z9112 revealed 16 and 39 flanking recombinant embryos, respectively. The zero-recombinant region (light green, contained in two bacterial artificial chromosomes [BACs]) was refined with additional markers. Sequencing of med14 revealed a base pair substitution (arrow) leading to a premature stop codon () in each of the three log alleles.

(B–E′′′) Lateral and dorsal views of the otic vesicle and pectoral fin of 3.5 dpf WT (B–B′′′), log mutant (C–C′′′), med14 morpholino-injected (D–D′′′), and log mutant injected with med14 RNA (E–E′′′) embryos.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	med14^s231/s231 WT + MO1-med14
Knockdown Reagent:	MO1-med14
Observed In:	embryonic pectoral fin morphogenesis heart looping heart tube inner ear inner ear development pectoral fin semicircular canal
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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