FIGURE

Fig. 6

ID

ZDB-FIG-150512-6

Publication

Burgoyne et al., 2015 - Regulation of melanosome number, shape and movement in the zebrafish retinal pigment epithelium by OA1 and PMEL

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

PMELa MO prevents melanosome movement into the apical processes and has a profound effect on photoreceptor morphology. (A–D) Electron micrographs showing the effect on melanosome positioning in the apical processes at 5dpf for zebrafish injected with control (A), PMELa (B), PMELb (C) and PMELa&b MOs (D). (B,D) PMELa and PMELa&b MOs resulted in missing or disrupted outer segments as shown by the asterisks. Scale bars: 4µm. (E) Photoreceptors in fish injected with PMELa and PMELa&b MOs had significantly fewer cylindrical melanosomes at 5dpf. Ctrl, control. (F) PMELa and PMELa&b MOs resulted in a reduced area of melanin (thus fewer melanosomes) in the apical processes. Results show the mean±s.e.m.; *P<0.05, ***P<0.001 (Student′s t-test).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-pmelb WT + MO2-pmela WT + MO1-pmelb + MO2-pmela
Knockdown Reagents:	MO1-pmelb MO2-pmela
Observed In:	melanosome assembly melanosome localization photoreceptor cell photoreceptor outer segment retinal pigmented epithelium melanosome
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.