Fig. 5

Foxi1 and Gata3 are involved in PPR differentiation regulation after 6 hpf. A–F: Knocking down Gata3 function can restore the enlarged cxcr4b (B,C) and pitx3 (E,F) expression domains caused by the XAV939 (5–7 hpf) treatment, compared with those of control embryos (A,D). Double-stranded MO (gata3MO::gata3upcMO) is injected at the one-cell stage and cleaved by UV light at 6 hpf. G–L: Knocking down Foxi1 function can restore the enlarged pax2a (H,I) and sox3 (K,L) expression domains caused by the 3F8 (5–7 hpf) treatment, compared with those of control embryos (G,J). Double-stranded MO (foxi1MO::foxi1upcMO) is injected at the one-cell stage and cleaved by UV light at 6 hpf. Arrows indicate a corresponding organ or organ primordium of each embryo. A–F: Side views with anterior to the left; G–L: Dorsal views with anterior to the left. A–F: 24 hpf; G–I: 12 hpf; J–L: 11 hpf. Scale bar = 120 µm in A–F; 200 µm in G–L.