PUBLICATION

Dissecting the differentiation process of the pre-placodal ectoderm in zebrafish

Authors
Yao, D., Zhao, F., Wu, Y., Wang, J., Wei, D., Zhao, J., Zhu, Z., Liu, D.
ID
ZDB-PUB-140722-10
Date
2014
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   243(10): 1338-51 (Journal)
Registered Authors
Zhao, Feng
Keywords
Fgf8, Foxi1, Gata3, PPR, Wnt
MeSH Terms
  • Animals
  • Body Patterning*/genetics
  • Bone Morphogenetic Protein Receptors/physiology
  • Bone Morphogenetic Proteins/physiology
  • Cell Differentiation*/genetics
  • Ectoderm/embryology*
  • Embryo, Nonmammalian
  • Fibroblast Growth Factors/genetics
  • Fibroblast Growth Factors/physiology
  • Gastrula/embryology
  • Gastrula/metabolism
  • Signal Transduction/genetics
  • Wnt Signaling Pathway/genetics
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology
(all 17)
PubMed
25044691 Full text @ Dev. Dyn.
Abstract
Background The preplacodal region (PPR) is a region of specialized ectoderm at the border of neural and nonneural ectoderm (NNE). Coordinated Bmp, Fgf, and Wnt signals are known to drive PPR development; however, the underlying mechanism is unknown.
Results We identified key components involved in PPR differentiation. The mesoderm/marginal Wnts at the early gastrula stage trigger differentiation by allowing the adjacent NNE border cells to start adopting caudal PPR fates; otherwise, the development of caudal PPR identity is hindered due to the persistent presence of gata3 mRNA. The caudal PPR fate dominates when foxi1 expression is enhanced at the late gastrula stage, and depleting Foxi1 after 6 hours postfertilization (hpf) reduces the otic-epibranchial placodal domain. When the Gata3 level is manipulated at the fertilized egg stage or near 6 hpf, the lens is always affected. In establishing PPR polarity, both Gata3 and Foxi1 inhibit Bmp signaling, whereas Foxi1 inhibits, but Gata3 enhances, Fgf sensitivity of the PPR cells.
Conclusions Our study reveals that in zebrafish, (1) the PPR at the shield stage may enter a developmental state when the PPR cells preferentially adopt a particular placodal fate and (2) a network of genetically linked factors, including Wnt/beta-catenin, Fgfr, Bmp, Gata3, and Foxi1, direct the process of PPR differentiation.
Genes / Markers
Marker Marker Type Name
bmp2bGENEbone morphogenetic protein 2b
bmp4GENEbone morphogenetic protein 4
bmperGENEBMP binding endothelial regulator
cldnaGENEclaudin a
cxcr4bGENEchemokine (C-X-C motif), receptor 4b
dlx3bGENEdistal-less homeobox 3b
egr2bGENEearly growth response 2b
fgf3GENEfibroblast growth factor 3
fgf8aGENEfibroblast growth factor 8a
fgfr1aGENEfibroblast growth factor receptor 1a
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Figures
Figure Gallery (7 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
TUchemical treatment: Wnt signalling activator75%-epiboly
TUchemical treatment: Wnt signalling activator1-4 somites
TUchemical treatment: Wnt signalling activator1-4 somites
TUchemical treatment: Wnt signalling activator1-4 somites
TUchemical treatment: Wnt signalling activator1-4 somites
TUchemical treatment: Wnt signalling activator1-4 somites
TUchemical treatment: Wnt signalling activator1-4 somites
TUchemical treatment: Wnt signalling activator1-4 somites
TUchemical treatment: Wnt signalling activator1-4 somites
TUchemical treatment: Wnt signalling activator5-9 somites
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
em1
    		Indel
    hi3747TgTransgenic Insertion
    ti282a
      		Point Mutation
      zf568TgTransgenic Insertion
        zf569TgTransgenic Insertion
          1 - 5 of 5
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          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          fgf3MO5-fgf3MRPHLNO
          foxi1MO2-foxi1MRPHLNO
          gata3MO1-gata3MRPHLNO
          tp53MO4-tp53MRPHLNO
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          Fish
          Fish
          TU
          TU + MO1-gata3
          TU + MO2-foxi1
          zf568Tg
          zf569Tg
          1 - 5 of 5
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          Antibodies
          No data available
          Orthology
          No data available
          Engineered Foreign Genes
          Marker Marker Type Name
          EGFPEFGEGFP
          1 - 1 of 1
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          Mapping
          No data available
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          Citation
          Yao, D., Zhao, F., Wu, Y., Wang, J., Wei, D., Zhao, J., Zhu, Z., Liu, D. (2014) Dissecting the differentiation process of the pre-placodal ectoderm in zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 243(10):1338-51.