Fig. 4

Normal Fin Development in fam38a Mutant Embryos

(A) Schematic of Fam38a and truncated Fam38a^um136. Light blue bars, transmembrane domains; DUF, domain of unknown function.

(B and C) Whole mount in situ hybridization of fam38a in (B) wild-type and (C) fam38a^um136 mutant at 28 hrpf.

(D) Schematic of fam38a exon 8 with um136 lesion and genotyping of individual embryo heads at 72 hrpf from an in-cross of fam38a^um136 heterozygous carriers. Homozygous mutants and heterozygotes pooled for subsequent RT-PCR are indicated by M and het, respectively.

(E) Schematic of exons in fam38a transcript (numbers in each box indicate exon number, followed by exon size).

(F) RT-PCR analysis of fam38a transcript in heterozygous and fam38a^um136 mutant trunks pooled from the het and M embryos in (D).

(G) Top five sequences are directly from PCR products from genomic DNA of numbered individuals in (D). HpyCH4III site is underlined and the 5-bp um136 deletion is evident. Bottom six sequences are cloned fragments from RT-PCR of pooled fam38a^um136 mutant embryos (embryos 7, 13, and 15).

(H and I) Transmitted light images of the tail fin fold in live (H) wild-type and (I) fam38a^um136 mutant embryos at 72 hrpf.