Fig. 2

Normal ISV Development in Selected Mutant Embryos

(A–F) Confocal micrographs of Tg(fli1a:egfp)^y1 or Tg(kdrl:egfp)^s843 transgenic embryos at 32 hrpf subjected to immunostaining with GFP antibody. (A) Wild-type sibling: ISVs, DLAV, and DA are indicated. (B–F) Embryos mutant for (B) arhgef9b^um45, (C) fgd5a^um134, (D) kalrnb^um80, (E) fmnl3^um150, or (F) pdgfrb^um148. Lateral views, anterior to the left, and dorsal is up.

(G) Whole mount in situ hybridization analysis of fmnl3 wild-type and fmnl3^um150 mutant sibling at 26 hrpf. Lateral views, anterior to the left, and dorsal is up.

(H) Schematic of fmnl3 locus flanking um150 allele and genotyping of individual embryo heads at 72 hrpf from an in-cross of fmnl3^um150 heterozygous carriers. Homozygous mutants and heterozygotes pooled for subsequent RT-PCR are indicated by M and het, respectively.

(I) Schematic of fmnl3 transcript flanking um150 and RT-PCR analysis of trunks from pooled M and het embryos shown in (H).

(J) Whole mount in situ hybridization analysis of pdgfrb in wild-type and pdgfrb^um148 mutant at 72 hrpf. Lateral views, anterior to the left, and dorsal is up.

(K) Western blot analysis of Pdgfrb and Egfp in lysates from Tg(fli1a:egfp)^y1;pdgfrb^um148 of indicated genotype at 72 hrpf.

(L) Schematic of ets1 exon 3 bearing the um206 lesion and genotyping of individual embryo heads at 3 days postfertilization from an in-cross of ets1^um206 heterozygous carriers.

(M) Schematic of ets1 transcript and RT-PCR analysis of trunks from pooled M and het embryos shown in (L).