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Fig. 3 Reduction of genes involved in the shh-related gene regulatory cassette in developing pectoral fin mesenchyme of Nipbl-deficient embryos.
Expression of fin mesenchymal genes at indicated stages in control and Nipbl-deficient embryos examined by ISH. Dorsal views, anterior to the top. (A, B) Expression of shha and ptch2 in pectoral fin buds (arrows) was significantly reduced in Nipbl-deficient embryos, while midline, neural tube expression was unaffected. Expression in endoderm derived-tissues (asterisks) is also reduced. (C) Expression of hand2 was also significantly reduced in stage-matched pectoral fin buds. hand2 expression in the fin buds and posterior lateral plate mesoderm is marked by brackets and arrowheads, respectively. (D) gli3 expression was not significantly affected in a stage-matched comparison.