FIGURE

Fig. 3

ID

ZDB-FIG-140811-39

Publication

Zhu et al., 2014 - Haploinsufficiency of Def Activates p53-Dependent TGFbeta Signalling and Causes Scar Formation after Partial Hepatectomy

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Fig. 3

Def haploinsufficiency results in a failure in the remodelling of the wound epidermis to liver tissue.

(A) Ventral view of the gross morphology of an amputated adult wild-type and def^+/-liver 7 days after PH. The dotted white line indicates the amputation site. 1: ventral lobe, 2: left dorsal lobe, 3: right dorsal lobe. (B–D) Representative images of co-immunostaining of PCNA and Bhmt to compare the process of epithelialisation of the amputation site 2 days after PH (B), wound epidermis formation 3 days after PH (C), and wound epidermis remodelling 5.5 days after PH (D) between the wild-type and the def^+/- mutant. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). In each case or at each time-point, more than 10 sections from at least three wild-type or def^+/- mutant fish were examined. bc, blood clot; lv, liver tissue; we, wound epidermis. Scale bar: 5 mm (A) and 75 μm (B–D).

Expression Data

Antibody:	Ab1-bhmt
Fish:	WT utp25^hi429Tg/+
Condition:	physical alteration: anatomical structure
Anatomical Term:	hepatocyte
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	utp25^hi429Tg/+
Condition:	physical alteration: anatomical structure
Observed In:	liver tissue remodeling
Stage:	Adult

Phenotype Detail

Acknowledgments

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