FIGURE

Fig. S3

ID

ZDB-FIG-140716-15

Publication

Astin et al., 2014 - Vegfd can compensate for loss of Vegfc in zebrafish facial lymphatic sprouting

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Fig. S3

Microangiography and lymphangiography

(A) Confocal images of 2 dpf lyve1:egfp embryos injected with fluorescent beads into the bloodstream to highlight blood flow. Vessels that express lyve1 (green) and contain blood flow (magenta) are veins (PHS, CCV and PCeV) while blood-containing vessels that do not express lyve1 are arteries (LDA). n=3 for control and flt4 morphants (B) Confocal images of 5 dpf lyve1:dsred embryos injected with fluorescein dextran to highlight functional lymphatics (lymphangiography). Data is representative of n=3 for wild type and n=4 for vegfc^hu5055. The vegfc^hu5055 mutant has a reduction in functional lymphatics at 5 dpf. CCV – common cardinal vein, PHS – primary head sinus, PCeV – posterior cerebral vein, LDA – lateral dorsal aorta, FLS – facial lymphatic sprout, LFL – lateral facial lymphatic, OLV – otolithic lymphatic vessel, MFL – medial facial lymphatic. Scale bars: 50 μm.

Expression Data

Genes:	DsRed EGFP
Fish:	nz101Tg nz150Tg nz150Tg + MO3-flt4 vegfc^{hu5055/hu5055}; nz101Tg
Knockdown Reagent:	MO3-flt4
Anatomical Terms:	common cardinal vein cranial lymph vessel facial lymphatic vessel facial lymphatic vessel lateral region facial lymphatic vessel lymphangiogenic sprout posterior cerebral vein primary head sinus
Stage Range:	Long-pec to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	vegfc^{hu5055/hu5055}; nz101Tg
Observed In:	cranial vasculature lymphatic system
Stage:	Day 5

Phenotype Detail

Acknowledgments

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