FIGURE

Fig. S2

ID

ZDB-FIG-140716-14

Publication

Astin et al., 2014 - Vegfd can compensate for loss of Vegfc in zebrafish facial lymphatic sprouting

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Fig. S2

flt4, ccbe1 and vegfc are required for secondary sprouting from the PCV

(A-D) Confocal images of 1.5 dpf lyve1:egfp embryos treated with (A) control MO (B) flt4 MO (C) ccbe1 MO (D) vegfc MO-1. Secondary sprouts from the PCV (arrowheads) are only present in embryos injected with control MO. (E) Quantitation of secondary sprout formation at 1.5 dpf in embryos injected with morpholinos. *** p<0.001, by a Mann-Whitney test to control; error bars indicate s.d. Scale bar: 50 μm.

Expression Data

Gene:	EGFP
Fish:	nz150Tg nz150Tg + MO1-ccbe1 nz150Tg + MO3-flt4 nz150Tg + MO3-vegfc
Knockdown Reagents:	MO1-ccbe1 MO3-flt4 MO3-vegfc
Anatomical Terms:	posterior cardinal vein posterior cardinal vein lymphangiogenic sprout
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nz150Tg + MO1-ccbe1 nz150Tg + MO3-flt4 nz150Tg + MO3-vegfc
Knockdown Reagents:	MO1-ccbe1 MO3-flt4 MO3-vegfc
Observed In:	lymphangiogenic sprout posterior cardinal vein
Stage:	Prim-25

Phenotype Detail

Acknowledgments

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