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Fig. S3
Microangiography and lymphangiography
(A) Confocal images of 2 dpf lyve1:egfp embryos injected with fluorescent beads into the bloodstream to highlight blood flow. Vessels that express lyve1 (green) and contain blood flow (magenta) are veins (PHS, CCV and PCeV) while blood-containing vessels that do not express lyve1 are arteries (LDA). n=3 for control and flt4 morphants (B) Confocal images of 5 dpf lyve1:dsred embryos injected with fluorescein dextran to highlight functional lymphatics (lymphangiography). Data is representative of n=3 for wild type and n=4 for vegfchu5055. The vegfchu5055 mutant has a reduction in functional lymphatics at 5 dpf. CCV – common cardinal vein, PHS – primary head sinus, PCeV – posterior cerebral vein, LDA – lateral dorsal aorta, FLS – facial lymphatic sprout, LFL – lateral facial lymphatic, OLV – otolithic lymphatic vessel, MFL – medial facial lymphatic. Scale bars: 50 μm.
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