FIGURE

Fig. 4

ID

ZDB-FIG-140715-59

Publication

Mackereth et al., 2005 - Zebrafish pax8 is required for otic placode induction and plays a redundant role with Pax2 genes in the maintenance of the otic placode

Other Figures

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Fig. 4

Interaction of pax8 with fgf3 and fgf8. Wild-type embryos (A-D), wild-type embryos injected with fgf3-MO (E-H), wild-type embryos co-injected with fgf3-MO and pax8-MO (I-L), ace (fgf8) mutants (M-P), and ace mutants injected with pax8-MO (Q-T). Images show lateral views of the otic vesicle at 30 hpf (A,E,I,M,Q), and dorsal views of pax8 expression at 10 hpf (B,F,J,N,R), pax2a expression at 12 hpf (C,G,K,O,S) and dlx3b expression at 12 hpf (D,H,L,P,T). op, otic placode. Arrowheads mark the otic region where dlx3b expression normally shows marked upregulation. Anterior is to the left in all specimens. Op, optic placode. Scale bar in T: 30 μm for A,E,I,M; 200 μm for B-D,F-H,J-L,N-P,R-T.

Expression Data

Genes:	dlx3b pax2a pax8
Fish:	WT fgf8a^{ti282a/ti282a} WT + MO1-fgf3 + MO2-fgf3 fgf8a^{ti282a/ti282a} + MO6-pax8 + MO7-pax8 WT + MO1-fgf3 + MO2-fgf3 + MO6-pax8 + MO7-pax8
Knockdown Reagents:	MO1-fgf3 MO2-fgf3 MO6-pax8 MO7-pax8
Anatomical Term:	otic placode
Stage Range:	Bud to 5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fgf8a^{ti282a/ti282a} WT + MO1-fgf3 + MO2-fgf3 fgf8a^{ti282a/ti282a} + MO6-pax8 + MO7-pax8 WT + MO1-fgf3 + MO2-fgf3 + MO6-pax8 + MO7-pax8
Knockdown Reagents:	MO1-fgf3 MO2-fgf3 MO6-pax8 MO7-pax8
Observed In:	hair cell otic placode otic vesicle otic vesicle morphogenesis otolith
Stage Range:	Bud to Prim-15

Phenotype Detail

Acknowledgments

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