FIGURE

Fig. 8

ID

ZDB-FIG-140325-69

Publication

Maurya et al., 2013 - Positive and negative regulation of Gli activity by Kif7 in the zebrafish embryo

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Fig. 8

Hh target gene regulation in the neural tube is largely independent of Kif7 and Sufu function.

(A–L) Lateral view at the level of yolk extension of 30 hpf WT (A,E,I), MZkif7 (B,F,J), ptch1;ptch2 double mutants (C,G,K) and sufu MO injected MZkif7 mutants (D,H,L) hybridized with antisense probes for fkd4 (A–D), nkx2.2a (E–H) or olig2 (I–L); Note the ventrally restricted domains of fkd4, olig2 and nkx2.2a typical of WT embryos are dramatically expanded in ptch1;ptch2 double mutant embryos (C,G,K) but are unaffected in the absence of Kif7 function. sufu MO injected MZkif7 embryos show wild-type patterns of nkx2.2a or olig2 but ectopically expression fkd4 in scattered cells. (M,N) expression of olig2 is completely lost from the neural tube in smo mutant embryos (M) but is partially restored by removal of kif7 function (N). Scale bar: 50 μm.

Expression Data

Genes:	foxa nkx2.2a olig2
Fish:	WT kif7^i271/i271 smo^b641/b641 kif7^i271/i271 + MO1-sufu ptch1^tj222/tj222; ptch2^{hu1602/hu1602} smo^b641/b641; kif7^i271/i271 (all 6)
Knockdown Reagent:	MO1-sufu
Anatomical Terms:	spinal cord spinal cord ventral region
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	smo^b641/b641 kif7^i271/i271 + MO1-sufu ptch1^tj222/tj222; ptch2^{hu1602/hu1602} smo^b641/b641; kif7^i271/i271
Knockdown Reagent:	MO1-sufu
Observed In:	spinal cord spinal cord ventral region
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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