Fig. 1

Targeted mutation of the zebrafish kif7 gene.

(A) Schematic representation of the nucleotide sequence in exon 3 of the zebrafish kif7 gene targeted by the Zinc finger nucleases. (B) Schematic representation of the human and zebrafish Kif7 coding sequences showing conserved exonic structure (dark shading) corresponding to different protein domains (drawn to scale). The black arrows indicate the approximate position of homozygous viable mutations found in some human patients and of the induced lesions in the zebrafish gene. (C,D) Expression of eng2a:gfp reporter gene in muscle fibers in the tail somites of wild-type (C) and kif7 homozygous (D) embryos at 2.5 dpf. Low level ectopic expression of the reporter is detected in fibers surrounding the muscle pioneers in the kif7 mutants. Merged images showing fast twitch muscle fibers stained with mAb F310 (red) are shown in the right-hand panels. Scale bar: 50 μm.