FIGURE

Fig. 1

ID

ZDB-FIG-140304-5

Publication

Su et al., 2014 - Cerebellar development in the absence of Gbx function in zebrafish

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Fig. 1

Gbx1 and Gbx2 function redundantly in cerebellum development. (A) Schematic of nonsense mutations identified in zebrafish gbx1 and gbx2 by TILLING. Both mutations are expected to prevent DNA binding by truncating the homeodomain. (B–I) Dorsal views at 22 hpf (B–E) or 6 dpf (F–I), anterior to the left. Genotypes are shown at the top. otx2 (blue), eng2b (red), and egr2b (blue) are expressed in midbrain, midbrain–hindbrain boundary (MHB), and rhombmere 3/5, respectively, in wildtype (WT), single and double mutants as shown. Tg(ptf1a:EGFP) (green) marks Purkinje neuron progenitors, Tg(olig2:DsRed2) (red) marks projection neurons, and anti-Vglut1/Slc17a7 (blue) marks cerebellar granule neuron axons. In gbx1^fh271;gbx2^fh253 double mutants the midbrain is expanded at the expense of r1 and no cerebellum forms.

Expression Data

Genes:	DsRed2 EGFP egr2b en2b otx2b
Antibody:	Ab1-slc17a7
Fish:	WT gbx1^fh271/fh271 gbx2^fh253/fh253 jh1Tg; vu19Tg gbx1^fh271/fh271; jh1Tg; vu19Tg gbx2^fh253/fh253; jh1Tg; vu19Tg gbx1^fh271/fh271; gbx2^fh253/fh253 gbx1^fh271/fh271; gbx2^fh253/fh253; jh1Tg; vu19Tg
Anatomical Terms:	cerebellar granule cell axon midbrain midbrain posterior region neuron neuron projection Purkinje cell rhombomere 1 rhombomere 3 rhombomere 5
Stage Range:	26+ somites to Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gbx1^fh271/fh271; gbx2^fh253/fh253; jh1Tg; vu19Tg
Observed In:	cerebellum cerebellum development
Stage:	Day 6

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 386(1), Su, C.Y., Kemp, H.A., and Moens, C.B., Cerebellar development in the absence of Gbx function in zebrafish, 181-90, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.