FIGURE

Fig. 4

ID

ZDB-FIG-140304-8

Publication

Su et al., 2014 - Cerebellar development in the absence of Gbx function in zebrafish

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Fig. 4

Rescue of the MHB program in gbx-embryos with otx knock-down. RNA in situ hybridization with genes shown on left; genotypes indicated at the top. Dorsal views of 22 hpf embryos with anterior to the left. (A–T) fgf8a, il17rd/sef, gbx2, pax2a and eng1b are all expressed at or around the MHB, are absent or strongly reduced in gbx- but are expanded in both otxMO and gbx-;otxMO embryos. (U–X) wnt1 is normally expressed in a narrow domain anterior to the MHB (U). This expression is reduced in gbx- (V) but rescued anterior to the extended isthmic region in otxMO (W) and gbx-;otxMO (X).

Expression Data

Genes:	en1b fgf8a gbx2 il17rd pax2a wnt1 (all 6)
Fish:	WT WT + MO1-otx2a + MO1-otx2b gbx1^fh271/fh271; gbx2^fh253/fh253 gbx1^fh271/fh271; gbx2^fh253/fh253 + MO1-otx2a + MO1-otx2b
Knockdown Reagents:	MO1-otx2a MO1-otx2b
Anatomical Term:	midbrain hindbrain boundary
Stage:	26+ somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 386(1), Su, C.Y., Kemp, H.A., and Moens, C.B., Cerebellar development in the absence of Gbx function in zebrafish, 181-90, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.