Fig. 1

Silencing across Gal4FF bicistronic transgenic insertions. (A) Lateral views of GFP and mCherry labeling in 3 dpf larvae from two transgenic lines in which GFP is regulated by the 4Xnr UAS. GFP expression recapitulates the pattern of mCherry in independently derived F1 larvae, which can be variable in their fluorescence. Representative sibling larvae in the F2 generation show widespread, highly mosaic, or largely absent mCherry and GFP labeling. (B) Colocalization of variegated mCherry and GFP fluorescence in muscle fibers of a c347 F2 larva. (C) Comparison of approximate number of GFP-labeled cells in F2 larvae from lines carrying the 4Xnr UAS (c342, c345, and c347) and the 14X UAS (c350). (D) Schematic of Gal4FF bipartite reporter construct and analysis of CpG methylation in c347 F2 larva from DNA bisulfite sequencing. Methylation at eleven CpGs within the EF1α promoter and the 4Xnr UAS are indicated on the horizontal axis, with black circles indicating methylated CpGs and open circles representing unmethylated CpGs. Patterns from eight different representative clones from one larva are shown on the vertical axis.