FIGURE

Fig. 8

ID

ZDB-FIG-131015-52

Publication

Wei et al., 2013 - miR-153 Regulates SNAP-25, Synaptic Transmission, and Neuronal Development

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Fig. 8

miR-153 regulates synaptic activity at the neuromuscular junction.

(A) FM1-43 loading of neuromuscular junction (NMJ) boutons in 55 hpf fish embryos. (B) Postsynaptic clusters of AChRs were labeled with Alexa 594-conjugated α-bungarotoxin. Overexpression of miR-153 caused decreased FM1-43 loading, indicating down-regulation of the synaptic vesicle cycle within NMJ boutons (arrowheads). (C) Knockdown of miR-153 (miR-153^MO) promoted greater uptake of FM1-43 dye, indicating increased synaptic vesicle cycling. Scale bar: 10 μm. (D) Quantification of FM1-43 fluorescent intensity with a paired Student’s t-test. Error bars show s.e.m. *p<0.01; **p<0.02.

Expression Data

Gene:	chrna1
Fish:	WT WT + MO1-dre-mir-153a + MO1-mir153
Knockdown Reagents:	MO1-dre-mir-153a MO1-mir153
Anatomical Terms:	myotome neuromuscular junction of somatic muscle skeletal muscle cell acetylcholine-gated channel complex
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-dre-mir-153a + MO1-mir153
Knockdown Reagents:	MO1-dre-mir-153a MO1-mir153
Observed In:	motor neuron synaptic vesicle endocytosis motor neuron synaptic vesicle exocytosis
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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