ZFIN Image: Wei et al., 2013, Fig. 8

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Figure Caption

Fig. 8

miR-153 regulates synaptic activity at the neuromuscular junction.

(A) FM1-43 loading of neuromuscular junction (NMJ) boutons in 55 hpf fish embryos. (B) Postsynaptic clusters of AChRs were labeled with Alexa 594-conjugated α-bungarotoxin. Overexpression of miR-153 caused decreased FM1-43 loading, indicating down-regulation of the synaptic vesicle cycle within NMJ boutons (arrowheads). (C) Knockdown of miR-153 (miR-153^MO) promoted greater uptake of FM1-43 dye, indicating increased synaptic vesicle cycling. Scale bar: 10 μm. (D) Quantification of FM1-43 fluorescent intensity with a paired Student’s t-test. Error bars show s.e.m. *p<0.01; **p<0.02.

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Acknowledgments

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