FIGURE

Fig. 4

ID

ZDB-FIG-131015-48

Publication

Wei et al., 2013 - miR-153 Regulates SNAP-25, Synaptic Transmission, and Neuronal Development

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Fig. 4

miR-153 mimics the effects of BoNT A.

(A) Single cell embryos were injected as indicated and then at 27 hpf, exposed to Botulinum neurotoxin A (BoNT) for 30 minutes. After recovery for 1 hour, western blots were performed on embryo lysates using antibodies against SNAP-25 or α–tubulin. (B) Quantitation of SNAP-25 levels from A, n = 3. **, p<0.01 (C) Embryonic movement in the presence or absence of BoNT A. The number of twitches per minute was counted as in Fig. 1 for embryos treated as indicated. Significance was determined by comparing mock embryos to all other conditions using ANOVA with Dunnett’s post-test, n = 15. *, p<0.05.

Expression Data

Antibody:	Ab2-snap25
Fish:	WT WT + MO1-dre-mir-153a + MO1-mir153
Condition:	chemical treatment: Botulinum toxin type A
Knockdown Reagents:	MO1-dre-mir-153a MO1-mir153
Anatomical Term:	whole organism
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT WT + MO1-dre-mir-153a + MO1-mir153
Condition:	chemical treatment: Botulinum toxin type A
Knockdown Reagents:	MO1-dre-mir-153a MO1-mir153
Observed In:	musculoskeletal movement
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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