FIGURE

Fig. 4

ID

ZDB-FIG-130401-24

Publication

Kondrychyn et al., 2013 - Stretching morphogenesis of the roof plate and formation of the central canal

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Fig. 4

The roof plate formation in the mib mutant.

(A), Confocal images, lateral view of the spinal cord of mib mutant, 72 hpf. Dashed rectangular shows the magnified in (A2) region of the spinal cord with the absence of GFP-positive RP cells. (B, C), the orthogonal optical sections of confocal images of the spinal cord of mib mutant illustrate lack of the roof plate extension between 36 and 72 hpf. Immunofluorescent staining of the transverse sections of spinal cord using anti-GFP (green) and anti-GFAP (red) antibodies; wild-type embryo (D–F) and mib mutant (G–I).

Expression Data

Gene:	EGFP
Antibody:	Ab3-gfap
Fish:	sqet33Et sqet3310Et mib1^ta52b/ta52b; sqet33Et/+
Anatomical Terms:	floor plate spinal cord region lateral wall spinal cord roof plate cell projection roof plate spinal cord region spinal cord apical surface
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mib1^ta52b/ta52b; sqet33Et/+
Observed In:	central canal roof plate spinal cord region roof plate spinal cord region cell projection spinal cord development
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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