FIGURE

Fig. 3

ID

ZDB-FIG-130401-23

Publication

Kondrychyn et al., 2013 - Stretching morphogenesis of the roof plate and formation of the central canal

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Fig. 3

Conversion of primitive lumen into central canal.

(A–L), Contraction of opposed apical surfaces reflects recession of the primitive lumen into a central canal between 24 and 72 hpf. Immunofluorescent staining by a combination of anti-GFP with anti-β-catenin antibody (A–D), anti-ZO-1 antibody (E–H), and phalloidin that detects F-actin (I–L). Increase in cell number and build-up of axonal tracts (*), plastic transverse sections of the spinal cord (M–P). (Q–T), Schematics corresponding to (M–P) showing cell nuclei.

Expression Data

Gene:	EGFP
Antibody:	Ab1-ctnnb
Fish:	sqet33Et
Anatomical Terms:	central canal roof plate cell projection roof plate spinal cord region
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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