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Fig. 4 The roof plate formation in the mib mutant.
(A), Confocal images, lateral view of the spinal cord of mib mutant, 72 hpf. Dashed rectangular shows the magnified in (A2) region of the spinal cord with the absence of GFP-positive RP cells. (B, C), the orthogonal optical sections of confocal images of the spinal cord of mib mutant illustrate lack of the roof plate extension between 36 and 72 hpf. Immunofluorescent staining of the transverse sections of spinal cord using anti-GFP (green) and anti-GFAP (red) antibodies; wild-type embryo (D–F) and mib mutant (G–I).
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