Fig. 6

Inhibition of the HMGCR pathway abolishes the intra-endothelial expression of cdc42. ((A) and (B)) Representative photomicrographs of Tg(fli1:EGFP-cdc42wt)^y48 embryos treated with DMSO or 1.0 mg/L of ATV and imaged at 48–52 hpf. ((C) and (D)) Representative photomicrographs of Tg(fli1:EGFP) embryos treated with DMSO or 1.0 mg/L of ATV and imaged at 48–52 hpf. Anterior is to the left and dorsal to the top. Scale bar=200 μm. ((E)–(H)) Graphs plotting relative EGFP signal intensities in the head and trunk vasculature (as denoted by the dotted white boxes in (A)–(D)) of (fli1:EGFP-cdc42wt)^y48 and Tg(fli1:EGFP) embryos treated with DMSO or 0.5 mg/L of ATV. Data are presented as the mean and standard error of the mean. N=15 embryos, t-test, p<0.001 (for (E) and (F)). ((I) and (J)) Representative composite confocal Z-stack projections of the trunk region of Tg(fli1:EGFP-cdc42wt)^y48 embryos, showing the localization of EGFP-cdc42 in a single intersegmental artery (ISV). The green arrow denotes perivacuolar expression of EGFP-cdc42. Scale bar=40 μm. Anterior is to the left and dorsal to the top.