FIGURE

Fig. 4

ID

ZDB-FIG-130318-7

Publication

Eisa-Beygi et al., 2013 - The 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR) pathway regulates developmental cerebral-vascular stability via prenylation-dependent signalling pathway

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Fig. 4

MO-mediated inhibition of the HMGCR pathway phenocopies ATV-treatment. (A) Partial hmgcrb pre-mRNA structure (not to scale) showing the exons (boxes), introns (black lines), the target of hmgcrb MO (red vertical arrow) and the positions of forward and reverse primers (black arrows) used to test the efficiency of the hmgcrb MO. ((B) and (C)) Representative bright-field micrographs of 48 hpf embryos injected with hmgcrb MO and control MO, stained with OD. Black arrows indicate sites of hemorrhage and blue arrow denotes the sinus venosus. Anterior is to the left and dorsal to the top. Scale bar=200 μm. (D) RT-PCR analysis of hmgcrb mRNA in hmgcrb morphant and control MO-injected embryos at 48 hpf. MO injection results in the dose-dependent appearance of a band corresponding to a transcript lacking exon-3. Doses of ATV are indicated above each lane.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-hmgcrb
Knockdown Reagent:	MO2-hmgcrb
Observed In:	brain pericardium nucleate erythrocyte
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 373(2), Eisa-Beygi, S., Hatch, G., Noble, S., Ekker, M., and Moon, T.W., The 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR) pathway regulates developmental cerebral-vascular stability via prenylation-dependent signalling pathway, 258-266, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.