FIGURE

Fig. 4

ID

ZDB-FIG-130123-68

Publication

Bhat et al., 2013 - A gene network that coordinates preplacodal competence and neural crest specification in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Unique requirements for foxi1 and gata3 in posterior placodes. Markers of cranial placodes and placodal derivatives, including Isl1 in the trigeminal placode at 14 hpf (A–C), pax2a in the otic/epibranchial placodes at 13 hpf (D-F, arrows), otic vesicle at 30 hpf (G-I), pitx3 in the anterior pituitary at 30 hpf (J-L, arrows) and the olfactory pit at 30 hpf (M–O, outlined) in control embryos (A, D, G, J and M), foxi1-gata3 morphants (B, E, H, K and N), and foxi1-gata3 morphants in which hs:tfap2a was activated at 7 hpf (C, F, I, L and O). Knockdown of foxi1 and gata3 reduces the size of the trigeminal, otic and epibranchial placodes, but anterior placodes develop normally. Activation of hs:tfap2a does not rescue development of posterior placodes in foxi1-gata3 morphants, and the transgene still enlarges anterior placodes despite knockdown of foxi1 and gata3. Images show lateral views with anterior to the right (A-C, G-I, M-O), dorsal views with anterior up (D-F), and facial views of the front of the head (J-L).

Expression Data

Genes:	pax2a pitx3
Fish:	WT WT + MO2-foxi1 + MO2-gata3 x24Tg + MO2-foxi1 + MO2-gata3
Condition:	heat shock
Knockdown Reagents:	MO2-foxi1 MO2-gata3
Anatomical Terms:	adenohypophyseal placode epibranchial field otic placode
Stage Range:	5-9 somites to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-foxi1 + MO2-gata3 x24Tg + MO2-foxi1 + MO2-gata3
Condition:	heat shock
Knockdown Reagents:	MO2-foxi1 MO2-gata3
Observed In:	adenohypophyseal placode olfactory pit otic placode otic vesicle trigeminal placode
Stage Range:	5-9 somites to Prim-15

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 373(1), Bhat, N., Kwon, H.J., and Riley, B.B., A gene network that coordinates preplacodal competence and neural crest specification in zebrafish, 107-117, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.