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Fig. 1

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ZDB-FIG-130123-65

Publication

Bhat et al., 2013 - A gene network that coordinates preplacodal competence and neural crest specification in zebrafish

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Fig. 1

Knockdown of tfap2a/c impairs early placode development. A–G, Expression of early placodal markers in control embryos and tfap2a/c morphants. Expression of pitx3 at 12.5 hpf marks pituitary/olfactory/lens precursors (A); cxcr4b marks the olfactory pit at 30 hpf (B); foxe3 marks the lens at 30 hpf (C); neuroD marks the trigeminal (tg) and anterior lateral line (al) at 14 hpf (D); pax8 at 10 hpf (E) and pax2a at 12 hpf (F) mark otic/epibranchial precursors; phox2a marks epibranchial ganglia (eg) at 30 hpf (G). H–J, Expression of competence factors foxi1 (H) and gata3 (I), and PPE marker dlx3b (J), at 8.5 hpf in control embryos and tfap2a/c morphants. K and L, Expression of tfap2a (K) and tfap2c (L) at 8.5 hpf in control embryos and tfap2a^-/- (lockjaw) mutants. M, otic vesicle at 30 hpf in a control embryo, tfap2a/c morphant, and embryos injected with sub-effective doses (0.5 ng) of foxi1-MO, dlx3b-MO or both foxi1-MO and dlx3b-MO. Sub-effective doses of these MO have little or no effect by themselves, but when combined have synergistic effects that phenocopy the tfap2a/c morphant. Images show dorsal views with anterior up (A, D–F), lateral views with anterior to the left (B, C, G, M) or lateral views with dorsal to the right (H–L).

Expression Data

Genes:	cxcr4b dlx3b foxe3 foxi1 gata3 neurod1 pax2a pax8 phox2a pitx3 tfap2a tfap2c
Fish:	WT tfap2a^ts213/ts213 WT + MO2-tfap2c + MO4-tfap2a
Knockdown Reagents:	MO2-tfap2c MO4-tfap2a
Anatomical Terms:	adenohypophyseal placode anterior lateral line system epibranchial ganglion lens lens placode olfactory field olfactory pit otic placode preplacodal ectoderm trigeminal placode
Stage Range:	75%-epiboly to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-dlx3b + MO2-foxi1 WT + MO2-tfap2c + MO4-tfap2a
Knockdown Reagents:	MO1-dlx3b MO2-foxi1 MO2-tfap2c MO4-tfap2a
Observed In:	adenohypophyseal placode anterior lateral line ectodermal placode development epibranchial ganglion lens lens placode olfactory field olfactory pit olfactory placode development otic placode otic placode formation otic vesicle trigeminal placode
Stage Range:	Bud to Prim-15

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 373(1), Bhat, N., Kwon, H.J., and Riley, B.B., A gene network that coordinates preplacodal competence and neural crest specification in zebrafish, 107-117, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.