Fig. S4
Parapineal differentiation is not dependent on migration. (A,B) 3D reconstructions of the pineal complex of DMSO- and 6 μM SU5402-treated larvae at 52 hpf. The parapineal (white arrowheads) fails to migrate away from the midline (dashed line) in SU5402-treated larvae. (C) Graph quantifying the number of sox1a-expressing cells. The number of sox1a-expressing cells is unchanged between DMSO- and SU5402-treated embryos. (D) Graph quantifying the distance of parapineal cell migration. Parapineal cell migration is greatly attenuated in SU5402-treated larvae (***P<0.0005 by t-test). Error bars represent s.e.m. n.s., not significant. Scale bar: 25 μm. |