FIGURE

Fig. S1

ID

ZDB-FIG-121205-5

Publication

Blaker-Lee et al., 2012 - Zebrafish homologs of 16p11.2, a genomic region associated with brain disorders, are active during brain development, and include two deletion dosage sensor genes

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Fig. S1

16p11.2 homologs are expressed during development and in the brain. (A) RNA expression analysis by RT-PCR. Total embryo RNA was analyzed for expression of 16p11.2 homologs at 2.5 hpf (blastula), 6 hpf (early gastrula), 11 hpf (late gastrula), 18 hpf (mid-somitogenesis), 24 hpf and 48 hpf. Most genes are expressed maternally, at 2.5 hpf, and expression persists zygotically (6 hpf and later). asphd1, doc2a, prrt2 and sez6L2 show zygotic expression only, suggesting that these genes act later in development. tbx24 expression declines sharply by 48 hpf, suggesting a narrow temporal activity window. Further, this gene is not expressed in brain. (8) Spatial expression analysis by whole mount in situ hybridization. Gene expression was analyzed at 24 hpf (see Methods and Table S4). (a-t) dorsal view, (a′-t′) lateral view. All genes except tbx24 were expressed in the brain.

Expression Data

Genes:	aldoaa asphd1 cdipt coro1a doc2a gdpd3a hirip3 ino80e kctd13 kif22 mapk3 maza mvp pagr1 ppp4ca prrt2 sez6l2 taok2a taok2b tbx6 tlcd3ba ypel3
Fish:	WT
Anatomical Terms:	brain whole organism
Stage Range:	256-cell to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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