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Fig. 2

ID

ZDB-FIG-121030-32

Publication

Hinits et al., 2012 - Zebrafish Mef2ca and Mef2cb are essential for both first and second heart field cardiomyocyte differentiation

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Fig. 2

Early cardiomyocytes fail to differentiate after loss of Mef2c function. A–F. In situ mRNA hybridisation for myl7, vmhc and smyd1b in wild type control (A), mef2ca^b1086+mef2cb MO (B), mef2ca^b1086 (C) mef2cb MO (D), mef2cb^fh288 (E) and mef2ca^b1086;mef2cb^fh288 (F) embryos, shown in wholemounts in dorsal view, anterior to top. Loss of both mef2ca and mef2cb function greatly reduces myl7, vmhc and smyd1b mRNAs in the bilateral heart fields (arrowheads; A,B and F). Mef2ca^b1086 mutant embryos have weak myl7 and smyd1b mRNAs early, but recover later, and show no change in vmhc (C). Mef2cb single morphants or mef2cb^fh288 mutant show no changes (D). G. Immunostaining for MyHC (A4.1025) in 24 ss mef2ca^b1086;mef2cb^fh288 embryos and their siblings, shown in wholemounts in dorsal view, anterior to top (left panel) and lateral view, anterior to left (right panel). No MyHC is detected in the heart, whereas somitic muscle appears normal (white arrowheads). Scale=100 μm.

Expression Data

Genes:	myh7 myl7 smyd1b
Antibody:	Ab-A4.1025
Fish:	WT mef2ca^b1086/b1086 mef2cb^fh288/fh288 WT + MO2-mef2cb mef2ca^b1086/b1086 + MO2-mef2cb mef2ca^b1086/+; mef2cb^fh288/+
Knockdown Reagent:	MO2-mef2cb
Anatomical Terms:	heart primordium heart rudiment somite
Stage Range:	10-13 somites to 20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mef2ca^b1086/b1086 mef2ca^b1086/b1086 + MO2-mef2cb mef2ca^b1086/+; mef2cb^fh288/+
Knockdown Reagent:	MO2-mef2cb
Observed In:	cardiac muscle cell cardiac muscle cell differentiation
Stage Range:	10-13 somites to 20-25 somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 369(2), Hinits, Y., Pan, L., Walker, C., Dowd, J., Moens, C.B., and Hughes, S.M., Zebrafish Mef2ca and Mef2cb are essential for both first and second heart field cardiomyocyte differentiation, 199-210, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.