FIGURE

Fig. S8

ID

ZDB-FIG-121030-29

Publication

Hinits et al., 2012 - Zebrafish Mef2ca and Mef2cb are essential for both first and second heart field cardiomyocyte differentiation

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Fig. S8

Mef2ca mutant has a normal heart.
A and B. Double mRNA in situ hybridisation for myl7 (red, A and B), vmhc (blue, A) and bmp4 (blue, B) in genotyped 48 hpf mef2ca mutant embryos and their siblings shown in ventral view, anterior to top. Mutants have normal looping and gene expression is no different than siblings. OFT, blue arrow, IFT, purple arrow, AVC, red arrows. C. Confocal stacks of 72 hpf mef2ca^b1086;Tg(myl7:GFP) mutants and their siblings and of Tg(myl7:GFP) injected with mef2ca MO immunostained for Elastin showing no difference between morphants, mutants and siblings. D. GFP+dsRED- CMs can only be found in OFT and IFT of the heart (dotted line) of mef2ca^b1086;Tg(myl7:GFP;myl7;ndsRed) mutants and their siblings at 60 hpf. E. Heart rate (bpm) of control wild type, mef2camorphants, mef2ca^tn213 and their siblings at 25-48 hpf. Embryos were grown in 24-well plates and genotype was determined at 5 dpf according to jaw phenotype (see Piotrowski et al., 1996). No significant differences according to t-test statistics were found at any stage between morphants/mutants and control/siblings. Number of embryos per condition is shown on columns. Variation in heart rate between experiments is due to variation in temperature. Scale = 100 μm.

Expression Data

Genes:	bmp4 DsRed2 EGFP elna myh7 myl7 (all 6)
Antibody:	Ab1-elna
Fish:	WT mef2ca^tn213/tn213 twu26Tg twu26Tg + MO1-mef2ca f2Tg; twu26Tg mef2ca^b1086/b1086; f2Tg; twu26Tg mef2ca^b1086/b1086; twu26Tg (all 7)
Knockdown Reagent:	MO1-mef2ca
Anatomical Terms:	atrioventricular canal atrium bulbus arteriosus bulbus arteriosus cardiac muscle cell cardiac ventricle heart sinus venosus sinus venosus cardiac muscle cell (all 8)
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mef2ca^tn213/tn213 WT + MO1-mef2ca
Knockdown Reagent:	MO1-mef2ca
Observed In:	heart heart contraction heart looping
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 369(2), Hinits, Y., Pan, L., Walker, C., Dowd, J., Moens, C.B., and Hughes, S.M., Zebrafish Mef2ca and Mef2cb are essential for both first and second heart field cardiomyocyte differentiation, 199-210, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.