FIGURE

Fig. 7

ID

ZDB-FIG-120522-21

Publication

Das et al., 2012 - Bmps and id2a act upstream of twist1 to restrict ectomesenchyme potential of the cranial neural crest

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Fig. 7

Bmp activity is selectively down-regulated in ectomesenchyme precursors.

(A) Confocal projection of pSMAD1/5/8 immunostaining at 12 hpf shows that all sox10:GFP-positive CNCCs display high pSMAD1/5/8. (B–D) At 16.5 hpf, a projection (B) and a high-magnification section (C, boxed area in B) show that pSMAD1/5/8 remains high in the dorsal non-ectomesenchyme but is lower in the more ventral ectomesenchyme. An orthogonal section (D, taken at the level of the line in C) shows that high pSMAD1/5/8 CNCCs remain in the neural epithelium whereas low pSMAD1/5/8 CNCCs are positioned more medial and ventral. The eye also displays high pSMAD1/5/8. Abbreviations: D, dorsal; V, ventral; A, anterior; P, posterior; M, medial; L, lateral; m, mandibular arch ectomesenchyme; h, hyoid arch ectomesenchyme; n, non-ectomesenchyme; e, ectomesenchyme; ve, ventral epithelium. Scale bar = 50 μm.

Expression Data

Gene:	GFP
Antibody:	Ab1-smad
Fish:	el8Tg
Anatomical Terms:	cranial neural crest ectomesenchyme neural tube ectoderm pharyngeal arch 1 ectomesenchyme pharyngeal arch 2 ectomesenchyme
Stage Range:	5-9 somites to 14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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