FIGURE

Fig. 9

ID

ZDB-FIG-120316-109

Publication

Kirchmaier et al., 2012 - The Popeye domain containing 2 (popdc2) gene in zebrafish is required for heart and skeletal muscle development

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Fig. 9

The hearts of popdc2 morphants display a variety of cardiac conduction defects. SPIM videos of the heart of zebrafish embryos injected with (A) control (CTR) and (B–D) MO1-popdc2 morpholino in the Tg(cmlc2:gCaMP)s878 background. The individual movies (see SI Movie 5, Movie 6, Movie 7 and Movie 8) were processed to determine the fluorescence intensity of selected regions of the heart over time. Each selected region has a corresponding number plotted below. (A) In a control (CTR), fluorescence intensity varies with time in atrial and ventricular regions as the depolarization wave propagates through the heart. (B) Heart displaying a single incidence (asterisks) of a 4:1 AV block. (C) Heart displaying alternating phases of normal conduction and 2:1 AV block (asterisks). Both hearts depicted in (B) and (C) also display irregular atrial conduction waves. (D) Example of a 6 dpf heart with sinoatrial block (asterisk).The hearts of popdc2 morphants display a variety of cardiac conduction defects. SPIM videos of the heart of zebrafish embryos injected with (A) control (CTR) and (B–D) MO1-popdc2 morpholino in the Tg(cmlc2:gCaMP)s878 background. The individual movies (see SI Movies 5–8) were processed to determine the fluorescence intensity of selected regions of the heart over time. Each selected region has a corresponding number plotted below. (A) In a control (CTR), fluorescence intensity varies with time in atrial and ventricular regions as the depolarization wave propagates through the heart. (B) Heart displaying a single incidence (asterisks) of a 4:1 AV block. (C) Heart displaying alternating phases of normal conduction and 2:1 AV block (asterisks). Both hearts depicted in (B) and (C) also display irregular atrial conduction waves. (D) Example of a 6 dpf heart with sinoatrial block (asterisk).

Expression Data

Gene:	EGFP
Fish:	s878Tg s878Tg + MO1-popdc2
Knockdown Reagent:	MO1-popdc2
Anatomical Terms:	atrium cardiac ventricle
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s878Tg + MO1-popdc2
Knockdown Reagent:	MO1-popdc2
Observed In:	cardiac conduction heart
Stage:	Day 5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 363(2), Kirchmaier, B.C., Poon, K.L., Schwerte, T., Huisken, J., Winkler, C., Jungblut, B., Stainier, D.Y., and Brand, T., The Popeye domain containing 2 (popdc2) gene in zebrafish is required for heart and skeletal muscle development, 438-450, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.