FIGURE

Fig. 4

ID

ZDB-FIG-120315-56

Publication

Petrey et al., 2012 - Excessive activity of cathepsin K is associated with the cartilage defects in a zebrafish model for mucolipidosis II

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

MMP activity is increased in ML-II embryos. (A–C) General MMP activity was measured in WT and ML-II embryo lysates across a developmental time course (1–4 dpf; A), following introduction of GNPTAB mRNA (3 dpf; B) and after incubation with EDTA (inhibits MMPs) or APMA (activates MMPs) for 2 hours (3 dpf; C). (D) Activity of MMP-12 and/or -13 in 3-dpf WT and ML-II embryos measured using specific fluorogenic substrate. (E) MMP-13 immunoblots show increased steady-state levels of both the MMP-13 precursor (48 kDa) and the mature active (35 kDa) forms in 3-dpf ML-II embryo lysates. β-tubulin was used as a loading control. (F) General MMP activity assays in isolated feline synovial fibroblast-like cell lysates. *P<0.05, **P<0.01, ***P<0.001, Student’s t-test. Activity measurements for all conditions were performed on at least three independent embryo lysates.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.